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article · Microbial Cell Factories · 2005

Screening for novel enzymes from metagenome and SIGEX, as a way to improve it

Jiae Yun, Sangryeol Ryu

Cited by 100Open access

Abstract

Metagenomics has been successfully applied to isolate novel biocatalysts from the uncultured microbiota in the environment. Two types of screening have been used to identify clones carrying desired traits from metagenomic libraries: function-based screening, and sequence-based screening. Both function- and sequence- based screening have individual advantages and disadvantages, and they have been applied successfully to discover biocatalysts from metagenome. However, both strategies are laborious and tedious because of the low frequency of screening hits. A recent paper introduced a high throughput screening strategy, termed substrate-induced gene-expression screening (SIGEX). SIGEX is designed to select the clones harboring catabolic genes induced by various substrates in concert with fluorescence activated cell sorting (FACS). This method was applied successfully to isolate aromatic hydrocarbon-induced genes from a metagenomic library. Although SIGEX has many limitations, it is expected to provide economic advantages, especially to industry.

Cite this paper

APA 7

Yun, J., & Ryu, S. (2005). Screening for novel enzymes from metagenome and SIGEX, as a way to improve it. Microbial Cell Factories, 4(1), 8. https://doi.org/10.1186/1475-2859-4-8

MLA 9

Yun, Jiae, and Sangryeol Ryu. “Screening for novel enzymes from metagenome and SIGEX, as a way to improve it.” Microbial Cell Factories, vol. 4, no. 1, 2005, p. 8, https://doi.org/10.1186/1475-2859-4-8.

Chicago

Yun, Jiae, and Sangryeol Ryu. 2005. “Screening for novel enzymes from metagenome and SIGEX, as a way to improve it.” Microbial Cell Factories 4 (1): 8. https://doi.org/10.1186/1475-2859-4-8.

Harvard

Yun, J. and Ryu, S. (2005) ‘Screening for novel enzymes from metagenome and SIGEX, as a way to improve it’, Microbial Cell Factories, 4(1), p. 8. Available at: https://doi.org/10.1186/1475-2859-4-8.

BibTeX
@article{yun2005screening,
  title = {{Screening for novel enzymes from metagenome and SIGEX, as a way to improve it}},
  author = {Yun, Jiae and Ryu, Sangryeol},
  journal = {Microbial Cell Factories},
  volume = {4},
  number = {1},
  pages = {8},
  year = {2005},
  doi = {10.1186/1475-2859-4-8}
}

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