article · The Analyst · 2003
Determination of trace concentrations of dissolved nitric oxide in a biological buffer
Eric Demoncheaux, Paul J. Foster, Colin Borland, Adrian Smith, T W Higenbottam, Michael B. Davies
Cited by 2Abstract and details only
Abstract
A new real-time method for measuring a trace concentration of nitric oxide (NO) in a complex matrix routinely used in pharmacological studies of its bioactivity is described. NO was quantified as a gas by chemiluminescence after extraction from a continuous liquid sample flow with a limit of detection of 0.042 nmol dm(-3) at a signal to noise ratio of 3. Theories to calculate the concentration of NO in the liquid sample flow from a direct measurement of NO in the extraction carrier gas are presented. The efficiency of extraction is determined by a stopflow experiment. An example is presented of the measurement of the steady-state concentrations of NO in Krebs-bicarbonate buffer at pH 7.4 and 37 degrees C when its liquid surface is sequentially exposed to gases containing various concentrations of NO in O2 plus CO2.
Cite this paper
Demoncheaux, E., Foster, P. J., Borland, C., Smith, A., Higenbottam, T. W., & Davies, M. B. (2003). Determination of trace concentrations of dissolved nitric oxide in a biological buffer. The Analyst, 128(10), 1281. https://doi.org/10.1039/b310188f
Demoncheaux, Eric, et al. “Determination of trace concentrations of dissolved nitric oxide in a biological buffer.” The Analyst, vol. 128, no. 10, 2003, p. 1281, https://doi.org/10.1039/b310188f.
Demoncheaux, Eric, Paul J. Foster, Colin Borland, Adrian Smith, T W Higenbottam, and Michael B. Davies. 2003. “Determination of trace concentrations of dissolved nitric oxide in a biological buffer.” The Analyst 128 (10): 1281. https://doi.org/10.1039/b310188f.
Demoncheaux, E. et al. (2003) ‘Determination of trace concentrations of dissolved nitric oxide in a biological buffer’, The Analyst, 128(10), p. 1281. Available at: https://doi.org/10.1039/b310188f.
@article{demoncheaux2003determination,
title = {{Determination of trace concentrations of dissolved nitric oxide in a biological buffer}},
author = {Demoncheaux, Eric and Foster, Paul J. and Borland, Colin and Smith, Adrian and Higenbottam, T W and Davies, Michael B.},
journal = {The Analyst},
volume = {128},
number = {10},
pages = {1281},
year = {2003},
doi = {10.1039/b310188f}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.