article · Analytical Chemistry · 2007
Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation
Zhengzheng Fei, Shengnian Wang, Yubing Xie, Brian E Henslee, Chee Guan Koh, L James Lee
Cited by 65Abstract and details only
Abstract
To avoid safety issues such as immune response and cytotoxicity associated with viruses and liposomes, physical methods have been widely used for either in vivo or ex vivo gene delivery. They are, however, very invasive and often provide limited efficiency. Using pEGFP and pSEAP plasmids and NIH 3T3 fibroblasts as models, we demonstrate a new electroporation-based gene delivery method, called membrane sandwich electroporation (MSE). The MSE method is able to provide better gene confinement near the cell surface to facilitate gene transport into the cells and thus shows significant improvement over transgene expression of mammalian cells compared to current electroporation techniques.
Cite this paper
Fei, Z., Wang, S., Xie, Y., Henslee, B. E., Koh, C. G., & Lee, L. J. (2007). Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation. Analytical Chemistry, 79(15), 5719–5722. https://doi.org/10.1021/ac070482y
Fei, Zhengzheng, et al. “Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation.” Analytical Chemistry, vol. 79, no. 15, 2007, pp. 5719–22, https://doi.org/10.1021/ac070482y.
Fei, Zhengzheng, Shengnian Wang, Yubing Xie, Brian E Henslee, Chee Guan Koh, and L James Lee. 2007. “Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation.” Analytical Chemistry 79 (15): 5719–22. https://doi.org/10.1021/ac070482y.
Fei, Z. et al. (2007) ‘Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation’, Analytical Chemistry, 79(15), pp. 5719–5722. Available at: https://doi.org/10.1021/ac070482y.
@article{fei2007gene,
title = {{Gene Transfection of Mammalian Cells Using Membrane Sandwich Electroporation}},
author = {Fei, Zhengzheng and Wang, Shengnian and Xie, Yubing and Henslee, Brian E and Koh, Chee Guan and Lee, L James},
journal = {Analytical Chemistry},
volume = {79},
number = {15},
pages = {5719--5722},
year = {2007},
doi = {10.1021/ac070482y}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.