article · FEMS Immunology & Medical Microbiology · 2006

Lactobacillusstrains isolated from the vaginal microbiota of healthy women inhibitPrevotella biviaandGardnerella vaginalisin coculture and cell culture

Fabrice Atassi, Dominique Brassart, Philipp Grob, Federico Graf, Alain L. Servin

Cited by 136Open access

Abstract

The purpose of this study was to investigate how human vaginal isolates of Lactobacillus acidophilus, Lactobacillus jensenii, Lactobacillus gasseri and Lactobacillus crispatus inhibit the vaginosis-associated pathogens Gardnerella vaginalis and Prevotella bivia. Results show that all the strains in coculture condition reduced the viability of G. vaginalis and P. bivia, but with differing degrees of efficacy. The treatment of G. vaginalis- and P. bivia-infected cultured human cervix epithelial HeLa cells with L. gasseri strain KS120.1 culture or cell-free culture supernatant (CFCS) results in the killing of the pathogens that are adhering to the cells. The mechanism of the killing activity is not attributable to low pH and the presence of lactic acid alone, but rather to the presence of hydrogen peroxide and proteolytic enzyme-resistant compound(s) present in the CFCSs. In addition, coculture of G. vaginalis or P. bivia with L. gasseri KS120.1 culture or KS120.1 bacteria results in inhibition of the adhesion of the pathogens onto HeLa cells.

Cite this paper

APA 7

Atassi, F., Brassart, D., Grob, P., Graf, F., & Servin, A. L. (2006). Lactobacillusstrains isolated from the vaginal microbiota of healthy women inhibitPrevotella biviaandGardnerella vaginalisin coculture and cell culture. FEMS Immunology & Medical Microbiology, 48(3), 424–432. https://doi.org/10.1111/j.1574-695x.2006.00162.x

MLA 9

Atassi, Fabrice, et al. “Lactobacillusstrains isolated from the vaginal microbiota of healthy women inhibitPrevotella biviaandGardnerella vaginalisin coculture and cell culture.” FEMS Immunology & Medical Microbiology, vol. 48, no. 3, 2006, pp. 424–32, https://doi.org/10.1111/j.1574-695x.2006.00162.x.

Chicago

Atassi, Fabrice, Dominique Brassart, Philipp Grob, Federico Graf, and Alain L. Servin. 2006. “Lactobacillusstrains isolated from the vaginal microbiota of healthy women inhibitPrevotella biviaandGardnerella vaginalisin coculture and cell culture.” FEMS Immunology & Medical Microbiology 48 (3): 424–32. https://doi.org/10.1111/j.1574-695x.2006.00162.x.

Harvard

Atassi, F. et al. (2006) ‘Lactobacillusstrains isolated from the vaginal microbiota of healthy women inhibitPrevotella biviaandGardnerella vaginalisin coculture and cell culture’, FEMS Immunology & Medical Microbiology, 48(3), pp. 424–432. Available at: https://doi.org/10.1111/j.1574-695x.2006.00162.x.

BibTeX

@article{atassi2006lactobacillus,
  title = {{<i>Lactobacillus</i>strains isolated from the vaginal microbiota of healthy women inhibit<i>Prevotella bivia</i>and<i>Gardnerella vaginalis</i>in coculture and cell culture}},
  author = {Atassi, Fabrice and Brassart, Dominique and Grob, Philipp and Graf, Federico and Servin, Alain L.},
  journal = {FEMS Immunology & Medical Microbiology},
  volume = {48},
  number = {3},
  pages = {424--432},
  year = {2006},
  doi = {10.1111/j.1574-695x.2006.00162.x}
}

Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.