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article · Methods in molecular biology · 2011

Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells

Sujoy K. Dhara, Anirban Majumder, Mahesh C. Dodla, Steven L. Stice

Cited by 9Abstract and details only

Abstract

Human pluripotent stem cells (hPSCs) have been used to derive self-renewing neural progenitor (NP) cell lines. Here we describe methods to genetically modify these cells. Detailed methods for transfection and nucleofection in PSC-derived NP cells are presented. We have shown that nucleofection results in higher yield of GFP(+) NP cells as compared with transfection. However, nucleofection leads to higher cell death than transfection. Application of these methods allows for the development of novel tools to study human development and cellular differentiation. Genetically modified NPs have direct application in neural imaging, tracking neural cells, and for drug delivery to target organs using neural progenitor cells as carriers.

Cite this paper

APA 7

Dhara, S. K., Majumder, A., Dodla, M. C., & Stice, S. L. (2011). Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells. Methods in molecular biology, 767, 343–354. https://doi.org/10.1007/978-1-61779-201-4_25

MLA 9

Dhara, Sujoy K., et al. “Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells.” Methods in molecular biology, vol. 767, 2011, pp. 343–54, https://doi.org/10.1007/978-1-61779-201-4_25.

Chicago

Dhara, Sujoy K., Anirban Majumder, Mahesh C. Dodla, and Steven L. Stice. 2011. “Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells.” Methods in molecular biology 767: 343–54. https://doi.org/10.1007/978-1-61779-201-4_25.

Harvard

Dhara, S.K. et al. (2011) ‘Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells’, Methods in molecular biology, 767, pp. 343–354. Available at: https://doi.org/10.1007/978-1-61779-201-4_25.

BibTeX
@article{dhara2011nonviral,
  title = {{Nonviral Gene Delivery in Neural Progenitors Derived from Human Pluripotent Stem Cells}},
  author = {Dhara, Sujoy K. and Majumder, Anirban and Dodla, Mahesh C. and Stice, Steven L.},
  journal = {Methods in molecular biology},
  volume = {767},
  pages = {343--354},
  year = {2011},
  doi = {10.1007/978-1-61779-201-4_25}
}

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