review · Biotechnology Journal · 2017
Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts
Marvin Kadisch, Christian Willrodt, M. Hillen, Bruno Bühler, Andreas Schmid
Cited by 41Abstract and details only
Abstract
A systematic and powerful knowledge-based framework exists for improving the activity and stability of chemical catalysts and for empowering the commercialization of respective processes. In contrast, corresponding biotechnological processes are still scarce and characterized by case-by-case development strategies. A systematic understanding of parameters affecting biocatalyst efficiency, that is, biocatalyst activity and stability, is essential for a rational generation of improved biocatalysts. Today, systematic approaches only exist for increasing the activity of whole-cell biocatalysts. They are still largely missing for whole-cell biocatalyst stability. In this review, we structure factors affecting biocatalyst stability and summarize existing, yet not completely exploited strategies to overcome respective limitations. The factors and mechanisms related to biocatalyst destabilization are discussed and demonstrated inter alia based on two case studies. The factors are similar for processes with different objectives regarding target molecule or metabolic pathway complexity and process scale, but are in turn highly interdependent. This review provides a systematic for the stabilization of whole-cell biocatalysts. In combination with our knowledge on strategies to improve biocatalyst activity, this paves the way for the rational design of superior recombinant whole-cell biocatalysts, which can then be employed in economically and ecologically competitive and sustainable bioprocesses.
Cite this paper
Kadisch, M., Willrodt, C., Hillen, M., Bühler, B., & Schmid, A. (2017). Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts. Biotechnology Journal, 12(8). https://doi.org/10.1002/biot.201600170
Kadisch, Marvin, et al. “Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts.” Biotechnology Journal, vol. 12, no. 8, 2017, https://doi.org/10.1002/biot.201600170.
Kadisch, Marvin, Christian Willrodt, M. Hillen, Bruno Bühler, and Andreas Schmid. 2017. “Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts.” Biotechnology Journal 12 (8). https://doi.org/10.1002/biot.201600170.
Kadisch, M. et al. (2017) ‘Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts’, Biotechnology Journal, 12(8). Available at: https://doi.org/10.1002/biot.201600170.
@article{kadisch2017maximizing,
title = {{Maximizing the stability of metabolic engineering‐derived whole‐cell biocatalysts}},
author = {Kadisch, Marvin and Willrodt, Christian and Hillen, M. and Bühler, Bruno and Schmid, Andreas},
journal = {Biotechnology Journal},
volume = {12},
number = {8},
year = {2017},
doi = {10.1002/biot.201600170}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.