article · Acta Biomaterialia · 2018
Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix
Majed Felemban, Birthe Dorgau, Nicola C. Hunt, Dean Hallam, Darin Zerti, Roman Bauer, Yuchun Ding, Joseph Collin, David Steel, Natalio Krasnogor, Jumana Y. Al‐Aama, Susan Lindsay, Carla Mellough, Majlinda Lako
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Abstract
The expression and the role of many extracellular matrix (ECM) components during human retinal development is not fully understood. In this study, expression of key ECM components (Collagen IV, Fibronectin, Brevican, Versican, IMPG1 and IMPG2) was investigated during human retinal ontogenesis. Collagen IV and Fibronectin were expressed in Bruch's membrane; whereas Brevican, Versican, IMPG1 & IMPG2 in the developing interphotoreceptor matrix (IPM). Retinal organoids were successfully generated from pluripotent stem cells. The expression of ECM components was examined in the retinal organoids and found to recapitulate human retinal development in vivo. Using functional blocking experiments, we were able to highlight an important role for IMPG1 and CD44 in the development of photoreceptors and IPM formation.
Cite this paper
Felemban, M., Dorgau, B., Hunt, N. C., Hallam, D., Zerti, D., Bauer, R., Ding, Y., Collin, J., Steel, D., Krasnogor, N., Al‐Aama, J. Y., Lindsay, S., Mellough, C., & Lako, M. (2018). Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. Acta Biomaterialia, 74, 207–221. https://doi.org/10.1016/j.actbio.2018.05.023
Felemban, Majed, et al. “Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix.” Acta Biomaterialia, vol. 74, 2018, pp. 207–21, https://doi.org/10.1016/j.actbio.2018.05.023.
Felemban, Majed, Birthe Dorgau, Nicola C. Hunt, Dean Hallam, Darin Zerti, Roman Bauer, Yuchun Ding, et al. 2018. “Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix.” Acta Biomaterialia 74: 207–21. https://doi.org/10.1016/j.actbio.2018.05.023.
Felemban, M. et al. (2018) ‘Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix’, Acta Biomaterialia, 74, pp. 207–221. Available at: https://doi.org/10.1016/j.actbio.2018.05.023.
@article{felemban2018extracellular,
title = {{Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix}},
author = {Felemban, Majed and Dorgau, Birthe and Hunt, Nicola C. and Hallam, Dean and Zerti, Darin and Bauer, Roman and Ding, Yuchun and Collin, Joseph and Steel, David and Krasnogor, Natalio and Al‐Aama, Jumana Y. and Lindsay, Susan and Mellough, Carla and Lako, Majlinda},
journal = {Acta Biomaterialia},
volume = {74},
pages = {207--221},
year = {2018},
doi = {10.1016/j.actbio.2018.05.023}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.