article · Ultrasonics Sonochemistry · 2021
The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli
Katherine M. Costello, Eirini Velliou, Jorge Gutierrez‐Merino, Cindy Smet, Hani El Kadri, Jan Van Impe, Madeleine Bussemaker
Cited by 42Open access
Abstract
Ultrasound, alone or in combination with natural antimicrobials, is a novel food processing technology of interest to replace traditional food decontamination methods, as it is milder than classical sterilisation (heat treatment) and maintains desirable sensory characteristics. However, ultrasound efficacy can be affected by food structure/composition, as well as the order in which combined treatments are applied. More specifically, treatments which target different cell components could result in enhanced inactivation if applied in the appropriate order. The microbial properties i.e. Gram positive/Gram negative can also impact the treatment efficacy. This work presents a systematic study of the combined effect of ultrasound and nisin on the inactivation of the bacteria Listeria innocua (Gram positive) and Escherichia coli (Gram negative), at a range of cavitation conditions (44, 500, 1000 kHz). The order of treatment application was varied, and the impact of system structure was also investigated by varying the concentration of Xanthan gum used to create the food model systems (0 - 0.5% w/v). Microbial inactivation kinetics were monitored, and advanced microscopy and flow cytometry techniques were utilised to quantify the impact of treatment on a cellular level. Ultrasound was shown to be effective against E. coli at 500 kHz only, with L. innocua demonstrating resistance to all frequencies studied. Enhanced inactivation of E. coli was observed for the combination of nisin and ultrasound at 500 kHz, but only when nisin was applied before ultrasound treatment. The system structure negatively impacted the inactivation efficacy. The combined effect of ultrasound and nisin on E. coli was attributed to short-lived destabilisation of the outer membrane as a result of sonication, allowing nisin to penetrate the cytoplasmic membrane and facilitate cell inactivation.
Cite this paper
Costello, K. M., Velliou, E., Gutierrez‐Merino, J., Smet, C., Kadri, H. E., Van Impe, J., & Bussemaker, M. (2021). The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli. Ultrasonics Sonochemistry, 79, 105776. https://doi.org/10.1016/j.ultsonch.2021.105776
Costello, Katherine M., et al. “The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli.” Ultrasonics Sonochemistry, vol. 79, 2021, p. 105776, https://doi.org/10.1016/j.ultsonch.2021.105776.
Costello, Katherine M., Eirini Velliou, Jorge Gutierrez‐Merino, Cindy Smet, Hani El Kadri, Jan Van Impe, and Madeleine Bussemaker. 2021. “The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli.” Ultrasonics Sonochemistry 79: 105776. https://doi.org/10.1016/j.ultsonch.2021.105776.
Costello, K.M. et al. (2021) ‘The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli’, Ultrasonics Sonochemistry, 79, p. 105776. Available at: https://doi.org/10.1016/j.ultsonch.2021.105776.
@article{costello2021effect,
title = {{The effect of ultrasound treatment in combination with nisin on the inactivation of Listeria innocua and Escherichia coli}},
author = {Costello, Katherine M. and Velliou, Eirini and Gutierrez‐Merino, Jorge and Smet, Cindy and Kadri, Hani El and Van Impe, Jan and Bussemaker, Madeleine},
journal = {Ultrasonics Sonochemistry},
volume = {79},
pages = {105776},
year = {2021},
doi = {10.1016/j.ultsonch.2021.105776}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.