Preparing feeder-free hPSCs for nucleofection v1

Abstract

This protocol describes the standard procedure preparing feeder-free human pluripotent stem cells (hPSCs) for nucleofection. General notes 1. Throughout this protocol, the term hPSC is used to collectively refer to both hiPSCs and hESCs. All described procedures have been tested and work equally well for hiPSCs and hESCs. 2. This protocol is to prepare cells for protocol nucleofection of hPSCs. Before starting, familiarize yourself with the protocol and the required preparations. A detailed protocol on maintaining MEF-cultured hPSCs can be found in the collection "Maintenance and inactivation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell culture;" doi: 3. Detailed protocols for preparing plasmids, RNA, and RNP for nucleofection can be found in the collection "Nucleofection (Amaxa) and electroporation (Biorad) of hPSCs." A link to this collection can be found in the title section of this protocol, located above.

Cite this paper

@misc{li2022preparing,
  title = {{Preparing feeder-free hPSCs for nucleofection v1}},
  author = {Li, Hanqin},
  year = {2022},
  doi = {10.17504/protocols.io.b4ptqvnn}
}

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