CD34+ Cell RNP Nucleofection v1

Abstract

Human CD34+hematopoietic stem and progenitor cells (HSPCs) are a standard source of cells for clinical HSC transplantations as well as experimental xenotransplantation to generate “humanized mice”. To further extend the range of applications of these humanized mice, we developed a protocol to efficiently edit the genomes of human CD34+HSPCs before transplantation. We have optimized nucleofection of sgRNA:Cas9 ribonucleoprotein complexes and are now able to edit a candidate gene in CD34+HSPCs with almost 100% efficiency, and without affecting their potential for engraftment and multilineage differentiation in mice.

Cite this paper

@misc{kuppers2022cd34,
  title = {{CD34+ Cell RNP Nucleofection v1}},
  author = {Kuppers, Daniel A.},
  year = {2022},
  doi = {10.17504/protocols.io.q26g7y541gwz/v1}
}

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