other · Figshare · 2024
Isolates selection algorithm.
Braulio Josué Méndez-Sotelo (18405243), Mónica Delgado-Beltrán (18405246), Melissa Hernández-Durán (7862702), Claudia Adriana Colín-Castro (18405249), José Esquivel-Bautista (18405252), Sandra Angélica Ortega-Oliva (18405255), Jossue Ortiz-Álvarez (17782875), Rodolfo García-Contreras (49039), Rafael Franco-Cendejas (6508286), Luis Esau Lopez Jacome (18405258)
Cited by 0Open access
Abstract
<div><p>Background</p><p>Infections caused by <i>Stenotrophomonas maltophilia</i> and related species are increasing worldwide. Unfortunately, treatment options are limited, whereas the antimicrobial resistance is increasing.</p><p>Methods</p><p>We included clinical isolates identified as <i>S</i>. <i>maltophilia</i> by VITEK 2 Compact. Ceftazidime/avibactam, meropenem/vaborbactam, imipenem/relebactam, cefiderocol, quinolones, and tetracycline family members were evaluated by broth microdilution method and compared with first-line treatment drugs. Minimum inhibitory concentrations (MICs) were reported for all antibiotics. We sequenced the Whole Genome of cefiderocol resistant strains (CRSs) and annotated their genes associated with cefiderocol resistance (GACR). Presumptive phylogenetic identification employing the 16S marker was performed.</p><p>Results</p><p>One hundred and one clinical strains were evaluated, sulfamethoxazole and trimethoprim, levofloxacin and minocycline showed susceptibilities of 99.01%, 95.04% and 100% respectively. Ceftazidime was the antibiotic with the highest percentage of resistance in all samples (77.22%). Five strains were resistant to cefiderocol exhibiting MIC values ≥ 2 μg/mL (4.95%). The β-lactamase inhibitors meropenem/vaborbactam and imipenem/relebactam, failed to inhibit <i>S</i>. <i>maltophilia</i>, preserving both MIC<sub>50</sub> and MIC<sub>90</sub> ≥64 μg/mL. Ceftazidime/avibactam restored the activity of ceftazidime decreasing the MIC range. Tigecycline had the lowest MIC range, MIC<sub>50</sub> and MIC<sub>90.</sub> Phylogeny based on 16S rRNA allowed to identify to cefiderocol resistant strains as putative species clustered into <i>Stenotrophomonas maltophilia</i> complex (Smc). In these strains, we detected GARCs such as Mutiple Drug Resistance (MDR) efflux pumps, L1-type β-lactamases, iron transporters and type-1 fimbriae.</p><p>Conclusion</p><p>Antimicrobial resistance to first-line treatment is low. The <i>in vitro</i> activity of new β-lactamase inhibitors against <i>S</i>. <i>maltophilia</i> is poor, but avibactam may be a potential option. Cefiderocol could be considered as a potential new option for multidrug resistant infections. Tetracyclines had the best <i>in vitro</i> activity of all antibiotics evaluated.</p></div>
Cite this paper
(18405243), B. J. M.-S., (18405246), M. D.-B., (7862702), M. H.-D., (18405249), C. A. C.-C., (18405252), J. E.-B., (18405255), S. A. O.-O., (17782875), J. O.-Á., (49039), R. G.-C., (6508286), R. F.-C., & (18405258), L. E. L. J. (2024). Isolates selection algorithm. Figshare. https://doi.org/10.1371/journal.pone.0298577.g001
(18405243), Braulio Josué Méndez-Sotelo, et al. “Isolates selection algorithm.” Figshare, 2024, https://doi.org/10.1371/journal.pone.0298577.g001.
(18405243), Braulio Josué Méndez-Sotelo, Mónica Delgado-Beltrán (18405246), Melissa Hernández-Durán (7862702), Claudia Adriana Colín-Castro (18405249), José Esquivel-Bautista (18405252), Sandra Angélica Ortega-Oliva (18405255), Jossue Ortiz-Álvarez (17782875), Rodolfo García-Contreras (49039), Rafael Franco-Cendejas (6508286), and Luis Esau Lopez Jacome (18405258). 2024. “Isolates selection algorithm.” Figshare. https://doi.org/10.1371/journal.pone.0298577.g001.
(18405243), B.J.M.-S. et al. (2024) ‘Isolates selection algorithm’, Figshare. Available at: https://doi.org/10.1371/journal.pone.0298577.g001.
@misc{184052432024isolates,
title = {{Isolates selection algorithm.}},
author = {(18405243), Braulio Josué Méndez-Sotelo and (18405246), Mónica Delgado-Beltrán and (7862702), Melissa Hernández-Durán and (18405249), Claudia Adriana Colín-Castro and (18405252), José Esquivel-Bautista and (18405255), Sandra Angélica Ortega-Oliva and (17782875), Jossue Ortiz-Álvarez and (49039), Rodolfo García-Contreras and (6508286), Rafael Franco-Cendejas and (18405258), Luis Esau Lopez Jacome},
journal = {Figshare},
year = {2024},
doi = {10.1371/journal.pone.0298577.g001}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.