other · Figshare · 2024

IHC negative control

Kanazawa, Yuji, Takahashi, Tatsuo, Inoue, Takao, Nagano, Mamoru, Koinuma, Satoshi, Eiyo, Haruki, Tamura, Yuma, Miyachi, Ryo, Iida, Naoya, Miyahara, Kenichiro, Shigeyoshi, Yasufumi

Cited by 0Open access

Abstract

Negative control experiments in immunohistochemistry. In morphological analysis by immunohistochemistry using anti-collagen I antibody, cross sections of tibialis anterior (TA) muscles were stained under identical staining conditions with the primary antibody subtracted as a negative control to eliminate the possibility of nonspecific antibody binding to the secondary antibody. Hematoxylin was used for counterstaining. Arrows, arrowheads and asterisks indicate the endomysium, perimysium regions, and muscle fiber(a-d). CTX: cardiotoxin. The right TA of old mice was designated as Old + CTX and the left TA as Old + Saline.

Cite this paper

APA 7

Kanazawa, Y., Takahashi, T., Inoue, T., Nagano, M., Koinuma, S., Eiyo, H., Tamura, Y., Miyachi, R., Iida, N., Miyahara, K., & Shigeyoshi, Y. (2024). IHC negative control. Figshare. https://doi.org/10.6084/m9.figshare.27274872.v3

MLA 9

Kanazawa, Yuji, et al. “IHC negative control.” Figshare, 2024, https://doi.org/10.6084/m9.figshare.27274872.v3.

Chicago

Kanazawa, Yuji, Tatsuo Takahashi, Takao Inoue, Mamoru Nagano, Satoshi Koinuma, Haruki Eiyo, Yuma Tamura, et al. 2024. “IHC negative control.” Figshare. https://doi.org/10.6084/m9.figshare.27274872.v3.

Harvard

Kanazawa, Y. et al. (2024) ‘IHC negative control’, Figshare. Available at: https://doi.org/10.6084/m9.figshare.27274872.v3.

BibTeX

@misc{kanazawa2024negative,
  title = {{IHC negative control}},
  author = {Kanazawa, Yuji and Takahashi, Tatsuo and Inoue, Takao and Nagano, Mamoru and Koinuma, Satoshi and Eiyo, Haruki and Tamura, Yuma and Miyachi, Ryo and Iida, Naoya and Miyahara, Kenichiro and Shigeyoshi, Yasufumi},
  journal = {Figshare},
  year = {2024},
  doi = {10.6084/m9.figshare.27274872.v3}
}

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