article · Figshare · 2016

Indirect Detection of Glycosidases Using Amperometry

BharatP. Gurale (2067166), Abasaheb N. Dhawane (2581501), Xikai Cui (2581498), Amrita Das (356600), Xiaohu Zhang (391063), Suri S. Iyer (1331244)

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Abstract

Glycosidases are essential enzymes\nthat cleave glycoside bonds.\nThe presence of glycosidases have been widely used to detect pathogens,\nlabel cells/tissues, and report specific diseases. We have developed\na rapid electrochemical assay to detect glycosidases. Exposure of\nelectrochemically inactive substrates to glycosidases releases glucose,\nwhich can be measured easily using an electrochemical cell. Five different\nglycosidases were detected rapidly within 1 h using disposable electrodes.\nThis assay could readily be incorporated into repurposed glucose meters\nto rapidly detect glycosidases, which in turn could be useful to report\nthe presence of a pathogen or illness.

Cite this paper

APA 7

(2067166), B. G., (2581501), A. N. D., (2581498), X. C., Das (356600), A., (391063), X. Z., & (1331244), S. S. I. (2016). Indirect Detection of Glycosidases Using Amperometry. Figshare. https://doi.org/10.1021/acs.analchem.5b03943.s001

MLA 9

(2067166), BharatP. Gurale, et al. “Indirect Detection of Glycosidases Using Amperometry.” Figshare, 2016, https://doi.org/10.1021/acs.analchem.5b03943.s001.

Chicago

(2067166), BharatP. Gurale, Abasaheb N. Dhawane (2581501), Xikai Cui (2581498), Amrita Das (356600), Xiaohu Zhang (391063), and Suri S. Iyer (1331244). 2016. “Indirect Detection of Glycosidases Using Amperometry.” Figshare. https://doi.org/10.1021/acs.analchem.5b03943.s001.

Harvard

(2067166), B.G. et al. (2016) ‘Indirect Detection of Glycosidases Using Amperometry’, Figshare. Available at: https://doi.org/10.1021/acs.analchem.5b03943.s001.

BibTeX

@article{20671662016indirect,
  title = {{Indirect Detection of Glycosidases Using Amperometry}},
  author = {(2067166), BharatP. Gurale and (2581501), Abasaheb N. Dhawane and (2581498), Xikai Cui and Das (356600), Amrita and (391063), Xiaohu Zhang and (1331244), Suri S. Iyer},
  journal = {Figshare},
  year = {2016},
  doi = {10.1021/acs.analchem.5b03943.s001}
}

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