other · Figshare · 2017

S1pr2 signalling attenuates Notch signalling.

Ioannis Serafimidis (3804352), Eva Rodriguez-Aznar (3804355), Mathias Lesche (2696755), Kazuaki Yoshioka (3010557), Yoh Takuwa (671994), Andreas Dahl (2696749), Duojia Pan (3804358), Anthony Gavalas (219589)

Cited by 0Open access

Abstract

<p>(A-F) Immunofluorescence analysis showed that Sel1l is strongly expressed in 14.5 dpc pancreata maintained for 1 d (14.5 dpc + 1 d) in ALI cultures compared to 14.5 dpc pancreata (A, D), and this correlated with a drop in the expression levels of NICD (B, E) and Hes1 (C, F). (G-I) S1pr2 signalling block with 15 μM JTE013 in 14.5 dpc + 1 d ALI cultures resulted in loss of Sel1l expression (G), concomitant with maintenance of high NICD (H) and Hes1 (I) expression. (J-L) Sel1l expression was restored in the absence of S1pr2 signalling by 1 μM of the specific proteasome inhibitor MG132 in 14.5 dpc + 1 d ALI cultures (J), and this was sufficient to restore attenuation of both NICD (K) and Hes1 (L) expression. Scale bars, 50μm (A-L). For raw data, please refer to the <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.2000949#pbio.2000949.s013" target="_blank">S1 Data</a> file.</p>

Cite this paper

APA 7

(3804352), I. S., (3804355), E. R.-A., (2696755), M. L., (3010557), K. Y., (671994), Y. T., (2696749), A. D., (3804358), D. P., & (219589), A. G. (2017). S1pr2 signalling attenuates Notch signalling. Figshare. https://doi.org/10.1371/journal.pbio.2000949.g008

MLA 9

(3804352), Ioannis Serafimidis, et al. “S1pr2 signalling attenuates Notch signalling.” Figshare, 2017, https://doi.org/10.1371/journal.pbio.2000949.g008.

Chicago

(3804352), Ioannis Serafimidis, Eva Rodriguez-Aznar (3804355), Mathias Lesche (2696755), Kazuaki Yoshioka (3010557), Yoh Takuwa (671994), Andreas Dahl (2696749), Duojia Pan (3804358), and Anthony Gavalas (219589). 2017. “S1pr2 signalling attenuates Notch signalling.” Figshare. https://doi.org/10.1371/journal.pbio.2000949.g008.

Harvard

(3804352), I.S. et al. (2017) ‘S1pr2 signalling attenuates Notch signalling’, Figshare. Available at: https://doi.org/10.1371/journal.pbio.2000949.g008.

BibTeX

@misc{38043522017s1pr2,
  title = {{S1pr2 signalling attenuates Notch signalling.}},
  author = {(3804352), Ioannis Serafimidis and (3804355), Eva Rodriguez-Aznar and (2696755), Mathias Lesche and (3010557), Kazuaki Yoshioka and (671994), Yoh Takuwa and (2696749), Andreas Dahl and (3804358), Duojia Pan and (219589), Anthony Gavalas},
  journal = {Figshare},
  year = {2017},
  doi = {10.1371/journal.pbio.2000949.g008}
}

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