other · Figshare · 2011

Fission yeast Hop2-Mnd1 stimulates D-loop formation by spDmc1

Ploquin, Mickaël, Petukhova, Galina V., Morneau, Dany, Déry, Ugo, Bransi, Ali, Stasiak, Andrzej, R. Daniel Camerini-Otero, Jean-Yves Masson

Cited by 0Open access

Abstract

<b>Copyright information:</b>Taken from "Stimulation of fission yeast and mouse Hop2-Mnd1 of the Dmc1 and Rad51 recombinases"Nucleic Acids Research 2007;35(8):2719-2733.Published online 10 Apr 2007PMCID:PMC1885673.© 2007 The Author(s) () Effect of spH2M1 in a buffer containing Mg (2.5 mM). Lane a, spDmc1 alone (4 μM); lanes b–f, effect of spH2M1 (0.25–4 μM) on spDmc1-mediated D-loop formation (4 μM); lane g, reaction with spH2M1 (4 μM) alone. () Effect of spH2M1 in a buffer containing Ca (2.5 mM). Lane a, DNA substrates (ssDNA and dsDNA) without proteins; lane b, spDmc1 alone (4 μM); lanes c–h, effect of spH2M1 (0.125–4 μM) on spDmc1-mediated D-loop formation (4 μM). () Analysis of the reversibility of the D-loop reaction by Dmc1 (4 μM) and spH2M1 (1 μM) in buffer containing Mg (2.5 mM). () Lane a, no protein (5 min reaction); lane b, no protein (60-min reaction); lanes c–i, reactions with spDmc1 and spH2M1 stopped at 1, 2.5, 5, 10, 20, 30 and 60 min, respectively; lanes j–p, spDmc1-mediated D-loop formation stopped at 1, 2.5, 5, 10, 20, 30 and 60 min, respectively. () Absence of reversibility of the D-loop reaction by spDmc1 (4 μM) and spH2M1 (1 μM) in buffer containing Ca (2.5 mM). Lane a, no protein (reaction was stopped immediately); lanes b–h, spDmc1-mediated D-loop formation stopped at 1, 2.5, 5, 10, 20, 30 and 60 min, respectively; lane i, no protein (60 min reaction); lanes j–p, reactions with spDmc1 and spH2M1 stopped at 1, 2.5, 5, 10, 20, 30 and 60 min, respectively. () Quantification of (), lanes b–g and (), lanes c–h, respectively. () Quantification of (), diamonds represent lanes c–i and squares correspond to lanes j–p. () Quantification of (), diamonds signify lanes j–p and squares correspond to lanes b–h.

Cite this paper

APA 7

Ploquin, M., Petukhova, G. V., Morneau, D., Déry, U., Bransi, A., Stasiak, A., Camerini-Otero, R. D., & Masson, J.-Y. (2011). Fission yeast Hop2-Mnd1 stimulates D-loop formation by spDmc1. Figshare. https://doi.org/10.6084/m9.figshare.22920.v1

MLA 9

Ploquin, Mickaël, et al. “Fission yeast Hop2-Mnd1 stimulates D-loop formation by spDmc1.” Figshare, 2011, https://doi.org/10.6084/m9.figshare.22920.v1.

Chicago

Ploquin, Mickaël, Galina V. Petukhova, Dany Morneau, Ugo Déry, Ali Bransi, Andrzej Stasiak, R. Daniel Camerini-Otero, and Jean-Yves Masson. 2011. “Fission yeast Hop2-Mnd1 stimulates D-loop formation by spDmc1.” Figshare. https://doi.org/10.6084/m9.figshare.22920.v1.

Harvard

Ploquin, M. et al. (2011) ‘Fission yeast Hop2-Mnd1 stimulates D-loop formation by spDmc1’, Figshare. Available at: https://doi.org/10.6084/m9.figshare.22920.v1.

BibTeX

@misc{ploquin2011fission,
  title = {{Fission yeast Hop2-Mnd1 stimulates D-loop formation by spDmc1}},
  author = {Ploquin, Mickaël and Petukhova, Galina V. and Morneau, Dany and Déry, Ugo and Bransi, Ali and Stasiak, Andrzej and Camerini-Otero, R. Daniel and Masson, Jean-Yves},
  journal = {Figshare},
  year = {2011},
  doi = {10.6084/m9.figshare.22920.v1}
}

Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.