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other · Figshare · 2015

Viral replication kinetics.

Karen Z. Lancaster (252215), Julie K. Pfeiffer (252217)

Cited by 0Open access

Abstract

<p>Mice were intramuscularly inoculated with 2×10<sup>7</sup> PFU of light-sensitive/neutral red poliovirus in the dark, tissues were harvested at 2, 6, 30 and 72 hpi in the dark, and virus was extracted in the dark. Half of the virus sample for each tissue was exposed to light (inactivating non-replicated viruses, to quantify replicated viruses), and half was maintained in the dark (to quantify all viruses). Virus in light-exposed and non-exposed samples was quantified by plaque assay. Total titer represents titer from non-light-exposed sample and is expressed as PFU/tissue (grey lines). Therefore, the grey lines represent titer from inoculum virus plus replicated virus. The percent of replicated viruses was obtained by dividing the light-exposed titer by the non-light-exposed titer for each individual sample (black bars). Mean with SEM from 3–7 mice is shown.</p>

Cite this paper

APA 7

(252215), K. Z. L., & (252217), J. K. P. (2015). Viral replication kinetics. Figshare. https://doi.org/10.1371/journal.ppat.1000791.g003

MLA 9

(252215), Karen Z. Lancaster, and Julie K. Pfeiffer (252217). “Viral replication kinetics.” Figshare, 2015, https://doi.org/10.1371/journal.ppat.1000791.g003.

Chicago

(252215), Karen Z. Lancaster, and Julie K. Pfeiffer (252217). 2015. “Viral replication kinetics.” Figshare. https://doi.org/10.1371/journal.ppat.1000791.g003.

Harvard

(252215), K.Z.L. and (252217), J.K.P. (2015) ‘Viral replication kinetics’, Figshare. Available at: https://doi.org/10.1371/journal.ppat.1000791.g003.

BibTeX
@misc{2522152015viral,
  title = {{Viral replication kinetics.}},
  author = {(252215), Karen Z. Lancaster and (252217), Julie K. Pfeiffer},
  journal = {Figshare},
  year = {2015},
  doi = {10.1371/journal.ppat.1000791.g003}
}

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