article · 2014
characterize biomarker colocalization
Xavier Moles Lopez, Paul Barbot, Yves-rémi Van Eycke, Laurine Verset, Lionel Larbanoix, Isabelle Salmon, Christine Decaestecker
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Abstract
Background and objective Extracting accurate information from complex biological processes involved in diseases, such as cancers, requires the simultaneous targeting of multiple proteins and locating their respective expression in tissue samples. This information can be collected by imaging and registering adjacent sections from the same tissue sample and stained by immunohistochemistry (IHC). Registration accuracy should be on the scale of a few cells to en-able protein colocalization to be assessed. Methods We propose a simple and efficient method based on the open-source elastix framework to register virtual slides of adjacent sections from the same tissue sample. We characterize registration accuracies for different types of tissue and IHC staining. Results Our results indicate that this technique is suitable for the evaluation of the colocalization of biomarkers on the scale of a few cells. We also show that using this technique in conjunction with a sequential IHC labeling and erasing technique offers improved registration accuracies. Discussion Brightfield IHC enables to address the problem of large series of tissue samples, which are usually required in clinical research. However, this approach, which is simple at the tissue processing level, requires challenging image analysis processes, such as accurate registration, to view and extract the protein colocalization information.
Cite this paper
Lopez, X. M., Barbot, P., Van Eycke, Y.-R., Verset, L., Larbanoix, L., Salmon, I., & Decaestecker, C. (2014). characterize biomarker colocalization.
Lopez, Xavier Moles, et al. “characterize biomarker colocalization.” 2014.
Lopez, Xavier Moles, Paul Barbot, Yves-rémi Van Eycke, Laurine Verset, Lionel Larbanoix, Isabelle Salmon, and Christine Decaestecker. 2014. “characterize biomarker colocalization.”
Lopez, X.M. et al. (2014) ‘characterize biomarker colocalization’.
@article{lopez2014characterize,
title = {{characterize biomarker colocalization}},
author = {Lopez, Xavier Moles and Barbot, Paul and Van Eycke, Yves-rémi and Verset, Laurine and Larbanoix, Lionel and Salmon, Isabelle and Decaestecker, Christine},
year = {2014}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.