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About 10,000 papers for “Bioanalysis”

Bioanalysis

Rizzi, A., Oefner, Peter J., Kenndler, E. · University of Regensburg Publication Server (University of Regensburg) · 2008

Cited by 0

Bioanalysis

Willem M. Albers, Arto Annila, Nicholas Goddard et al. · 2003

General Introduction Spectroscopy in the Biosensor and Genomics Age Genomics, Proteomics and Drug Discovery Biosensor Technologies Biomolecular Structure Determination Bioinformatics Optical Spectroscopy in Bioanalysis Introduction VIS/NIR Fluorescence Spectroscopy in DNA Sequencing and Immunoassay Bioanalytical Applications of Multi-photon Fluorescence Excitation (MPE) Bioluminescence, Chemiluminescence and Electrochemiluminescence Bioanalytical Applications of NIR Absorption Spectroscopy Bulk Optical Sensing Techniques Evanescent Wave Spectroscopy and Sensors Infrared and Raman Spectroscopy in Bioanalysis Circular Dichroism NMR Spectroscopy of Proteins Introduction Protein Sample Proton NMR Experiments Heteronuclear NMR Experiments Bioanalytical Mass Spectroscopy Introduction MALDI-TOF Electrospray Methods (ESI-MS) Tandem-MS TOF-SIMS MS in Protein Analysis MS in Nucleic Acid Analysis Conclusions

Cited by 0

Bioanalysis

Richard A. Scheltema · MPG.PuRe (Max Planck Society) · 2011

Cited by 0

Microscale Bioanalysis

Magnus Knutsson, Philip Timmerman, Frank A. Gomez · Bioanalysis · 2016

Over the last couple of years, tremendous technology

Cited by 2Open access

Bioanalysis 2010

Andreas Rizzi, Christopher Gerner · Electrophoresis · 2010

Cited by 0

Digital bioanalysis

Elizabeth M. Miller, Aaron R. Wheeler · Analytical and Bioanalytical Chemistry · 2008

Cited by 74

Focus on bioanalysis

Andreas Prange, B. Neidhart, G�nter Gauglitz · Analytical and Bioanalytical Chemistry · 2003

Cited by 0

Nanoparticle-Based Bioanalysis

Austin, John S. · Digital WPI · 2010

Using electrical impedance spectroscopy, three nanoparticle-based detection schemes were investigated. In particular, reproducibility and ease of amplification were thoroughly analyzed. Though all three configurations successfully detected the presence of the target molecule (human IgE), the sensor utilizing an aptamer sandwich mechanism generated reproducible, large impedance changes in the presence of human IgE.

Cited by 0Open access

Miniaturization in Bioanalysis

F. (František) Foret · ASEP · 2010

This presentation describes some of the new technology developments related to miniaturization, mass spectrometry coupling, sample enrichment and detection.

Cited by 0

Photoproteins in Bioanalysis

2006

Preface. List of Contributors. 1 The Photoproteins (Osamu Shimomura). 1.1 Discovery of Photoprotein. 1.2 Various Types of Photoproteins Presently Known. 1.2.1 Radiolarian (Protozoa) Photoproteins. 1.2.2 Coelenterate Photoproteins. 1.2.3 Ctenophore Photoproteins. 1.2.4 Pholasin (Pholas Luciferin). 1.2.5 Chaetopterus Photoprotein. 1.2.6 Polynoidin. 1.2.7 Symplectin. 1.2.8 Luminodesmus Photoprotein. 1.2.9 Ophiopsila Photoprotein. 1.3 Basic Strategy of Extracting and Purifying Photoproteins. 1.4 The Photoprotein Aequorin. 1.4.1 Extraction and Purifi cation of Aequorin. 1.4.1.1 Hydrophobic Interaction Chromatography. 1.4.2 Properties of Aequorin. 1.4.2.1 Stability. 1.4.2.2 Freeze-drying. 1.4.3 Specifi city to Ca 2+ . 1.4.4 Luminescence of Aequorin by Substances Other Than Divalent Cations. 1.4.5 Mechanism of Aequorin Luminescence and Regeneration of Aequorin. 1.4.5.1 Structure of Aequorin. 1.4.5.2 Luminescence Reaction. 1.4.5.3 Regeneration. 1.4.6 Inhibitors of Aequorin Luminescence. 1.4.7 Recombinant Aequorin. 1.4.8 Semi-synthetic Aequorins. 1.4.8.1 e-Aequorins. References. 2 Luminous Marine Organisms (Steven H.D. Haddock). 2.1 Introduction. 2.1.1 Non-luminous Taxa. 2.1.2 Luminous Taxa. 2.2 Taxonomic Distribution of Bioluminescence. 2.2.1 Bacterial Luminescence. 2.2.2 Dinofl agellate Luciferin. 2.2.3 Cypridina (Vargula) Luciferin. 2.2.4 Coelenterazine. 2.2.5 Other Luciferins: Known and Unknown. 2.3 Functions. 2.3.1 Startle or Distract. 2.3.2 Burglar Alarm. 2.3.3 Counterillumination. 2.3.4 Mating Displays. 2.3.5 Prey Attraction. References. 3 Beetle Luciferases: Colorful Lights on Biological Processes and Diseases (Vadim R. Viviani and Yoshihiro Ohmiya). 3.1 Introduction. 3.2 Beetle Luciferases. 3.3 Bioanalytical Assays of ATP. 3.3.1 Biomass Estimation and Microbiological Contamination. 3.3.2 Cytotoxicity and Cell Viability Tests. 3.3.3 Enzymatic Assays. 3.4 Luciferases as Reporter Genes. 3.4.1 Dual and Multiple Reporter Assays. 3.5 Biophotonic Imaging in Animals: A Living Light on Diseases. 3.5.1 Pathogen Infection in Living Models. 3.5.2 Drug Screening. 3.5.3 Tumor Proliferation and Regression Studies. 3.5.4 Gene Delivery and Gene Therapy. 3.5.5 Luciferase as Biomarkers for Cell Traffi cking Studies. 3.5.6 Immunoassays. 3.6 Biophotonic Imaging in Plants. 3.7 Biosensors: Sensing the Environment. 3.8 Novel Luciferases: Different Colors for Different Occasions. References. 4 Split Luciferase Systems for Detecting Protein-Protein Interactions in Mammalian Cells Based on Protein Splicing and Protein Complementation (Yoshio Umezawa). 4.1 Introduction. 4.2 Protein Splicing-based Split Firefl y Luciferase System [23]. 4.2.1 Split Luciferase Works as a Probe for Protein Interaction. 4.3 Split Renilla Luciferase Complementation System [33]. 4.3.1 Time Course of the Interaction Between Y941 and SH2n. 4.3.2 Location of the Interaction Between Y941 and SH2n. References. 5 Photoproteins in Nucleic Acid Analysis (Theodore K. Christopoulos, Penelope C. Ioannou, and Monique Verhaegen). 5.1 Hybridization Assays. 5.2 Quantitative Polymerase Chain Reaction. 5.3 Genotyping of Single-nucleotide Polymorphisms. 5.4 Conjugation of Aequorin to Oligodeoxynucleotide Probes. 5.5 Development of New Recombinant Bioluminescent Reporters. 5.6 Signal Amplifi cation by in Vitro Expression of DNA Reporters Encoding Bioluminescent Proteins. 5.7 Conclusions. References. 6 Bioluminescence Resonance Energy Transfer in Bioanalysis (Suresh Shrestha and Sapna K. Deo). 6.1 Introduction. 6.2 BRET Principle, Effi ciency, and Instrumentation. 6.3 Comparison of BRET and FRET. 6.4 Examples of BRET Donor-Acceptor Pairs. 6.5 Applications of BRET in Bioanalysis. 6.5.1 Homogeneous Assays. 6.5.2 Protein-Protein Interactions and High-throughput Screening. 6.6 Conclusions. References. 7 Photoproteins as in Vivo Indicators of Biological Function (Rajesh Shinde, Hui Zhao, and Christopher H. Contag). 7.1 Overview. 7.2 Probes Used for in Vivo Bioluminescence Imaging. 7.3 Probes Used for in Vivo Fluorescence Imaging. 7.4 Detection Technologies. 7.5 Current Applications. 7.5.1 Oncology. 7.5.2 Infectious Disease. 7.5.3 Bacterial Infections. 7.5.4 Viral Infections. 7.5.5 Viral-mediated Gene Transfer. 7.5.6 Cell Biology. 7.5.7 Stem Cell Biology. 7.6 Protease Sensors. 7.7 Conclusions. References. 8 Photoproteins as Reporters in Whole-cell Sensing (Jessika Feliciano, Patrizia Pasini, Sapna K. Deo, and Sylvia Daunert). 8.1 Introduction. 8.1.1 Biosensors Using Intact Cells. 8.1.2 Reporter Genes in Genetically Engineered Whole-cell Sensors. 8.2 The Luciferases. 8.2.1 Bacterial Luciferases. 8.2.1.1 luxAB Bioreporters. 8.2.1.2 luxCDABE Bioreporters. 8.2.1.3 Naturally Luminescent Bioreporters. 8.2.2 Eukaryotic Luciferases. 8.2.2.1 Firefl y Luciferase. 8.2.2.2 Sea Pansy Luciferase. 8.3 Aequorin. 8.4 Fluorescent Proteins. 8.4.1 Green Fluorescent Protein. 8.4.2 Red Fluorescent Protein. 8.5 Multiplexing. 8.6 Applications. 8.6.1 Stress Factors and Genotoxicants. 8.6.2 Environmental Pollutants. 8.6.3 Quorum-sensing Signaling Molecules. 8.6.4 Antibiotics. 8.7 Technological Advances. References. 9 Luminescent Proteins in Binding Assays (Aldo Roda, Massimo Guardigli, Elisa Michelini, Mara Mirasoli, and Patrizia Pasini). 9.1 Introduction. 9.2 Protein-Protein and Protein-Ligand Interaction Assays. 9.2.1 FRET and BRET Techniques. 9.2.2 FRET and BRET Applications. 9.2.3 Other Detection Principles. 9.3 Antibody-based Binding Assays. 9.3.1 Chemical Conjugation. 9.3.2 Gene Fusion. 9.3.3 Dual-analyte Assays. 9.3.4 Expression Immunoassays. 9.3.5 BRET-based Immunoassays. 9.4 Biotin-Avidin Binding Assays. 9.5 Nucleic Acid Hybridization Assays. 9.6 Other Binding Assays. 9.7 Concluding Remarks. References. 10 Luminescent Proteins: Applications in Microfl uidics and Miniaturized Analytical Systems (Emre Dikici, Laura Rowe, Elizabeth A. Moschou, Anna Rothert, Sapna K. Deo, and Sylvia Daunert). 10.1 Miniaturization and Microfl uidics. 10.2 Photoproteins and Applications in Miniaturized Detection Systems. 10.2.1 Green Fluorescent Protein. 10.2.1.1 GFP in Miniaturized Microfl uidic-based Assays. 10.2.2 Luciferase. 10.2.2.1 Luciferase in Miniaturized Microfl uidic-based Assays. 10.2.3 Aequorin. 10.2.3.1 Aequorin in Miniaturized Microfl uidic-based Assays. 10.3 Future Perspectives. References. 11 Advances in Instrumentation for Detecting Low-level Bioluminescence and Fluorescence (Eric Karplus). 11.1 Introduction. 11.2 Low Light Levels. 11.3 Methods of Coupling the Signal to the Detector. 11.3.1 Proximity Focusing. 11.3.2 Microscope Objectives. 11.3.3 Macro Lenses. 11.3.4 Fiber Optics. 11.4 Evaluating the Performance of an Optical System. 11.4.1 Numerical Aperture. 11.4.2 Transmission Effi ciency. 11.4.3 Magnifi cation. 11.5 Detector Technologies. 11.6 Selecting the Right Detector. 11.7 Detector Sensitivity. 11.8 Detector Noise. 11.9 Statistics of Photon Counting. 11.10 Summary. References. 12 Photoproteins and Instrumentation: Their Availability and Applications in Bioanalysis (Leslie Doleman, Stephanie Bachas-Daunert, Logan Davies, Sapna K. Deo, and Sylvia Daunert). Aequorin. Obelin. Luciferases. Aequorea and Anthozoa Fluorescent Proteins. Coelenteraziness. Luminometers. Fluorometers. Portable Luminometers. Disclaimer. Subject Index.

Cited by 66

Ligand (In Bioanalysis)

Ján Labuda, Richard P. Bowater, Miroslav Fojta et al. · IUPAC Standards Online · 2019

Recommendations are given concerning the terminology of methods of bioanalytical chemistry. With respect to dynamic development particularly in the analysis and investigation of biomacromolecules, terms related to bioanalytical samples, enzymatic methods, immunoanalytical methods, methods used in genomics and nucleic acid analysis, proteomics, metabolomics, glycomics, lipidomics, and biomolecules interaction studies are introduced.

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