RETURN TO ISSUEPREVNewsNEXTDNA ProfilingNew tool links evidence to suspects with high certaintyJOHN I. THORNTONCite this: Chem. Eng. News 1989, 67, 47, 18–30Publication Date (Print):November 20, 1989Publication History Published online10 November 2010Published inissue 20 November 1989https://pubs.acs.org/doi/10.1021/cen-v067n047.p018https://doi.org/10.1021/cen-v067n047.p018newsACS PublicationsCopyright © 1989 American Chemical SocietyArticle Views9Altmetric-Citations6LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access options SUBJECTS:Genetics Get e-Alerts

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DNA Profiling

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Recommendations are given concerning the terminology of methods of bioanalytical chemistry. With respect to dynamic development particularly in the analysis and investigation of biomacromolecules, terms related to bioanalytical samples, enzymatic methods, immunoanalytical methods, methods used in genomics and nucleic acid analysis, proteomics, metabolomics, glycomics, lipidomics, and biomolecules interaction studies are introduced.

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DNA Profiling

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This paper describes the process of DNA profiling, and its use in forensics and the courtroom. Beginning with the structure of DNA, it describes forensic procedures used, and DNA collection and extraction from samples. Also presented are some of the problems faced with presenting DNA as evidence in the courtroom, and a discussion of some of the landmark cases. It concludes with a discussion of the future possibilities of DNA profiling, and how it may be improved.

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We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template strand. A resulting distance alteration is reported by fluorescence resonance energy transfer between two dyes introduced into the molecular beacon stem. We describe real-time reaction profiling of two model DNA polymerases. We demonstrate kinetic characterization, rapid optimization of reaction conditions, and inhibitor profiling using the presented assay. Furthermore, to supersede purification steps in screening procedures of DNA polymerase mutant libraries, detection of enzymatic activity in bacterial expression lysates is described.

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