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About 10,000 papers for “Fluorescence microscope”

Fluorescence microscope

J. S. Ploem · AccessScience · 2015

AccessScience is an authoritative and dynamic online resource that contains incisively written, high-quality educational material covering all major scientific disciplines. An acclaimed gateway to scientific knowledge, AccessScience is continually expanding the ways it can demonstrate and explain core, trustworthy scientific information that inspires and guides users to deeper knowledge.

Cited by 0

Fluorescence microscope light source apparatus and fluorescence microscope

TAKAKI, RYOHEI, KABUKI, KIYOYUKI · Institutional Repository of Xi'an Institute of Optics and Fine Mechanics, Chinese Academy of Sciences (Xian Institute of Optics and Precision Mechanics) · 2018

Disclosed are a fluorescence microscope light source apparatus and a fluorescence microscope capable of obtaining high-luminance light in a wavelength of 500 to 550 nm and having reduced background noise when a sample is observed. The fluorescence microscope light source apparatus to be installed in a fluorescence microscope including an illumination light bandpass filter includes: a laser diode that emits blue light as excitation light; a phosphor that converts the excitation light from the laser diode into illumination fluorescence with a wavelength region of 500 to 550 nm; an optical system that extracts the illumination fluorescence from the phosphor; a first condenser lens that condenses the excitation light onto the phosphor; a light guide body having one end face on which the illumination fluorescence is incident and the other end face from which the illumination fluorescence exits; and a second condenser lens that condenses the illumination fluorescence onto the one end face of the light guide body. A band-elimination filter that blocks or attenuates light, out of the illumination fluorescence, in a wavelength region including a transmission maximum wavelength and including no transmission minimum wavelength in the illumination light bandpass filter is provided on a light path of the illumination fluorescence.

Cited by 0

Inverted Fluorescence Microscope

Makenzie Lyn Kamei, Enoch Wright Nicholson, Eduardo Reyna Miranda et al. · DigitalCommons - CalPoly (California State Polytechnic University) · 2020

The Inverted Fluorescence Microscope senior project team at Cal Poly, San Luis Obispo designed, assembled, and tested a proof-of-concept inverted fluorescence microscope for the university’s Microfabrication Laboratory. Administrators of the laboratory wished to use fluorescence for research and experiments involving cell growth and flow visualization on the micro-scale, and did not have the budget to purchase one of the costly commercially available options. The scope of this design challenge was to produce a low-cost inverted fluorescence microscope employing available optical components and additional readily sourced parts to expand the use of fluorescence microscopy accessible to undergraduate students in the Microfabrication Laboratory. This document is an account of the final microscope design as well as the engineering design process, project management procedures, and timeline followed to produce a working design verification prototype. The final product successfully resolved images of microfluidic devices in brightfield mode with automated maneuverability in the X-Y plane. It is equipped with fluorescence capabilities, and will serve as a valuable, low-cost research tool and platform for future student projects.

Cited by 0Open access

Inverted Fluorescence Microscope

Pfeiffer, Matthew B., Hann, Spencer George, Eggenberger, Thomas James et al. · DigitalCommons - CalPoly (California State Polytechnic University) · 2021

Team F13 is composed of Trevor Blythe, Spencer Hann, Matthew Pfeiffer, and Thomas Eggenberger. We are all majoring in mechanical engineering and in our final year of study here at Cal Poly San Luis Obispo. This project is a continuation of a 2019-2020 senior project. The previous team designed and built a functioning inverted fluorescence microscope (IFM) from scratch. This device was created as a lab tool for undergraduate students to be able to perform experiments on microfluidic devices constructed in Cal Poly’s Microfabrication Laboratory. Although substantially functional, several design constraints had not yet been met. Our team has improved microscope robustness and functionality for practical undergraduate lab use. To do this, we set overarching goals including decreasing microscope footprint, increasing the accuracy of microscope positional repeatability, and improving user-friendliness. Within this Final Design Review report, the full design, manufacturing, and testing processes of this project are explicitly detailed, as well as project logistics, future suggestions, and project management.

Cited by 0Open access

The Ploem Fluorescence Microscope

Guy Cox · 2019

Fluorescence microscopy was first introduced at the very beginning of the 20th century, and August Kohler was one of the pioneers. From the beginning of the twentieth century, great technological improvements were made in the fundamentally ancient technique of making glass filters. The driving forces were partly scientific, but the rapidly expanding popularity of photography also played a major part. From Ploem’s time up until the turn of the century the common light sources used in fluorescence microscopy were sealed high-pressure arc lamps. The recent development of high-intensity and short-wavelength light-emitting diodes has provided another alternative for fluorescence illuminators. Research-level fluorescence microscopes these days are often computer controlled, which tends to be great for the experienced user who can navigate the subtleties of the software. Computer control is the future, no doubt about it, but to get the best out of a microscope one must understand what is actually happening in the hardware.

Cited by 1

Portable fluorescence microscope system.

Alexander Golberg (515027), Gregory Linshiz (119206), Ilia Kravets (515028) et al. · Figshare · 2015

<p><b>A</b>) Microscope design scheme. <b>B</b>) Robotic stage and two cameras (arrows) for chip observation and data acquisition. <b>C</b>) ScanDrop system set up, including 2 pressure pumps to create droplets, a microscope imaging system, internet access, and a monitor for local data viewing.</p>

Cited by 0Open access

Chip-Scale Fluorescence Microscope

Efthymios P. Papageorgiou · eScholarship (California Digital Library) · 2019

CMOS image sensors have been widely used for decades, largely displacing CCD technology in cameras, scanners, telescopes, and a variety of medical sensors. Photodiodes are formed in the IC substrate and the technology has been repurposed to make more advanced structures with higher sensitivity, such as pinned or avalanche photodiodes. Less work has gone into generating optical elements using other features of the CMOS process, namely the metal interconnect. The precision deposition and spacing of the metal layers allows a variety of optical structures to be fabricated within the image sensor to perform both spatial and wavelength filtering. This is especially useful for applications where large-scale optical components, such as lenses or fiber optic bundles, cannot be used. For most intraoperative imaging applications and especially for modern minimally-invasive or robotic-assisted cancer surgery, these large optical elements restrict an imager's ability to thoroughly scan an entire tumor cavity.This thesis presents an image sensor incorporating angle-selective gratings for resolution enhancement in contact imaging applications. Optical structures designed in the CMOS metal layers above each photodiode form the angle-selective gratings that limit the sensor angle of view to plus or minus 18 degrees, rejecting background light and deblurring the image. The imager is based on a high-gain capacitive transimpedance amplifier pixel using a custom 11fF MOM capacitor, achieving 8.2V/s/pW sensitivity. The pixel also includes a leakage current minimization circuit to remove signal-dependent reset switch leakage and the corresponding dark current is 14aA/um^2. The resulting 4.7mm by 2.25mm sensor (80 by 36 pixels) is designed specifically for intraoperative cancer imaging in order to solve the pervasive challenge of identifying microscopic residual cancer foci in vivo. A custom amorphous silicon optical wavelength filter is used alongside the image sensor in order to perform fluorescence imaging. The filter rejects excitation light and has over five orders of magnitude rejection at 633nm. Fluorescence emission light above 700nm is allowed to transmit through. Several clinically-tested fluorophores, such as IR700DX, are compatible with this wavelength range. The filter is only 15um thick and is suitable for in vivo contact imaging applications as it maintains the same high rejection for all angles of incident light.We demonstrate imaging and detection of foci containing less than 200 cancer cells labeled with fluorescent biomarkers in 50ms and signal-to-noise ratios greater than 15dB using the custom image sensor and optical filter. We also demonstrate the detection of microscopic cancer using human tissue and residual tumor in mice models. The absence of large optical elements enables extreme miniaturization, allowing manipulation within a small, morphologically complex, tumor cavity.

Cited by 0Open access

A Scanning Apertureless Fluorescence Microscope

Guillaume Lessard Yang, T. J. Yang, Pierre Barritault et al. · 2007

We describe a near-field apertureless fluorescence microscope, capable of imaging fluorescent latex beads with subwavelength precision. The instrument is based on a home-built tapping-mode atomic-force microscope (AFM), to which an inverted optical microscope was added. The fact that the wavelength of the fluorescence that we observe is di#erent from the wavelength of the illumination allows for a relatively straightforward detection mechanism. Sample images are presented, along with evidence that the observed e#ect is of optical origin.

Cited by 0

Applications of Fluorescence Holographic Microscope

Zobač, Evžen · Brno University of Technology Digital Library (Brno University of Technology) · 2014

Tato bakalářská práce pojednává o fluorescenčním holografickém mikroskopu, interferometrickém mikroskopu s mimoosovou interferencí. Práce je rozdělena na část teoretickou a praktickou. V teoretické části je popsáno uspořádání fluorescenčního holografického mikroskopu a základní teorie ohledně fluorescenční interferometrie. Dále jsou zde uvedeny metody používané ke zvýšení rozlišení fluorescenčních mikroskopů. Praktická část se věnuje ověření zobrazovacích vlastností fluorescenčního holografického mikroskopu a také možnostem, které vylepší zobrazovací vlastnosti a měření jako takové.

Cited by 0Open access