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About 9,040 papers for “Video microscopy”

Video Microscopy

Borden, Jonathan Alan, Manuelidis, Laura · PubMed Central · 1988

Cited by 0Open access

Video microscopy

Dieter G. Weiss, Willi Maile, Robert A. Wick et al. · 1999

A new quality of microscopy, called video microscopy, emerges, if one observes the specimen instead with the human eye with a video camera connected to video processing equipment working at real time. Video microscopy is, therefore, much more then just adding a camera and monitor to the microscope to share the images with a larger audience. More recently, electronic devices other than video cameras, such as high sensitivity charge-coupled device

Cited by 0

Video Microscopy

Edward H. Hinchcliffe · Encyclopedia of Life Sciences · 2015

Abstract Video microscopy represents the first form of electronic imaging in light microscopy, and heralded the use of sophisticated fluorescence imaging techniques. However, today video microscopy – based on phase or differential interference optics – is considered to be an outdated technology. Rather than being yesterday's technology, a simple video microscope is a powerful tool for imaging cellular dynamics, and can easily be used to monitor cell cycle progression. Because the nonfluorescence imaging modes require significantly less light throughput, the video microscope is particularly suited for long‐term experiments, where photodamage can be a limiting factor. The video microscope is also an economical instrument that complements more sophisticated fluorescence‐based imaging systems, such as confocals, wide‐field deconvolution microscopes and super‐resolution microscopes. Here, I discuss a brief history of video microscopy, define some popular uses for the technology and discuss the selection of components for building a simple, yet functioning video microscopy system. Key Concepts Video microscopy represents the first electronic imaging system. Simple video microscopes are an economical choice for live‐cell imaging. System design requires selecting appropriate microscopes and illumination sources to allow long‐term imaging. Live‐cell imaging requires control of temperature and pH. Software selection to capture movies, control the imaging system and present the data is important.

Cited by 0

Video Microscopy

Borden, Jonathan Alan, Manuelidis, Laura · PubMed Central · 1988

Cited by 0Open access

Video microscopy

Sluder, Greenfield, Wolf, D. E., American Society for Cell Biology

Cited by 0

Video microscopy

Dieter G. Weiss, Willi Maile, Robert A Wick et al. · 1999

Abstract A new quality of microscopy, called video microscopy, emerges, if one observes the specimen, instead of with the human eye, with a video camera connected to video processing equipment working at real time. Video microscopy is, there fore, much more than just adding a camera and monitor to the microscope to share the images with a larger audience. More recently, electronic devices other than video cameras, such as high sensitivity charge coupled device (CCD) cameras and scanning light detector systems for confocal microscopy have been added to microscopes. The three fields (i) video-enhanced contrast microscopy for highest resolution work, (ii) video-intensified microscopy for low light applications, and (iii) electronic scanning microscopy for confocal microscopy and 3D imaging differ in the type of device generating the electronic image, but all three use basically the same types of analogue and digital image processors. While all these techniques are generally defined as electronic light microscopy, this chapter, video microscopy, deals with the first two techniques that involve CCD and video cameras as imaging devices. Video microscopy has produced a revolution in light microscopy of biological samples equivalent to that of the development of the immunofluorescence technique. It has once more made the traditional light microscope a powerful tool for those working on dynamic aspects of small biological systems, for example biochemists, molecular and cell biologists. It has given further resolving power to the light microscope enabling the observation of particles which bridge the size range between those normally studied by electron microscopy and those which are already well known to light microscopists as a whole, with the added advantage in that specimens can be examined alive. As well as allowing small particles to be resolved, the technique has the capacity to clean up the image, so allowing greater visibility. Also, changes of such parameters as amounts, concentrations, transport, or metabolism of specific molecules in both time and space can be quantitatively determined.

Cited by 5

Unsupervised Microscopy Video Denoising

Mary Aiyetigbo, Alexander Korte, Ethan M. Anderson et al. · 2024

In this paper, we introduce a novel unsupervised network to denoise microscopy videos featured by image sequences captured by a fixed location microscopy camera. Specifically, we propose a DeepTemporal Interpolation method, leveraging a temporal signal filter integrated into the bottom CNN layers, to restore microscopy videos corrupted by unknown noise types. Our unsupervised denoising architecture is distinguished by its ability to adapt to multiple noise conditions without the need for pre-existing noise distribution knowledge, addressing a significant challenge in real-world medical applications. Furthermore, we evaluate our denoising framework using both real microscopy recordings and simulated data, validating our outperforming video denoising performance across a broad spectrum of noise scenarios. Extensive experiments demonstrate that our unsupervised model consistently outperforms state-of-the-art supervised and unsupervised video denoising techniques, proving especially effective for microscopy videos. The project page is available at https://maryaiyetigbo.github.io/UMVD/

Cited by 2

Video Microscopy: The Fundamentals

Shinya Inoué, Kenneth R. Spring · 1986

Why Video? Microscope Image Formation. Practical Aspects of Microscopy. Physiological Characteristics of the Eye. Video Signal Fundamentals. Detection of Light and Vidicon Tube Cameras-Monochrome. Solid State Detectors and Cameras. Low-Light-Level Cameras. Color Video Signal and Color Cameras. Video Monitors, Projectors and Printers. Video Recorders. Digital Image Procesing. System Integration. Glossary of Terms. List of Manufacturers. Index.

Cited by 305

Temporality of video-microscopy.

Léa-Isabelle Renaud (18864456), Christophe Renaud (10294977), Samuel Delepoulle (10294974) et al. · Figshare · 2024

<p>Cells were plated 48,5 hours before the start of the video-microscopy; 24 hours after the plating, cells were treated with controls, chemotherapy or targeted treatment, the Sir-DNA was added 30min before the start of the experiment. After that, one picture in two channels (brightfield and far red) were taken each 10min during 72 hours. At the end of the 3rd day, the pictures were compiled into a movie.</p>

Cited by 0Open access

Progress in video microscopy

Shinya Inoué · Cell Motility and the Cytoskeleton · 1988

The progress in video microscopy is reviewed from its early inception, especially with respect to improvements of the microscope image quality. Very recent advances that provide serial optical sections and depth of field as thin as 0.1 micron and that make possible the recording of birefringent images of individual microtubules (25 nm in diameter) directly in live, dividing cells are also documented.

Cited by 21