article · Current Protocols in Protein Science · 2010

Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins

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Abstract

Analytical ultracentrifugation experiments play an integral role in the solution phase characterization of recombinant proteins and other biological macromolecules. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Optima XL-A or XL-I analytical ultracentrifuge. Optimal instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments.

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APA 7

Demeler, B. (2010). Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins. Current Protocols in Protein Science, 60(1), 7.13.1–7.13.24. https://doi.org/10.1002/0471140864.ps0713s60

MLA 9

Demeler, Borries. “Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins.” Current Protocols in Protein Science, vol. 60, no. 1, 2010, pp. 7.13.1–7.13.24, https://doi.org/10.1002/0471140864.ps0713s60.

Chicago

Demeler, Borries. 2010. “Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins.” Current Protocols in Protein Science 60 (1): 7.13.1–7.13.24. https://doi.org/10.1002/0471140864.ps0713s60.

Harvard

Demeler, B. (2010) ‘Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins’, Current Protocols in Protein Science, 60(1), pp. 7.13.1–7.13.24. Available at: https://doi.org/10.1002/0471140864.ps0713s60.

BibTeX

@article{demeler2010methods,
  title = {{Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins}},
  author = {Demeler, Borries},
  journal = {Current Protocols in Protein Science},
  volume = {60},
  number = {1},
  pages = {7.13.1--7.13.24},
  year = {2010},
  doi = {10.1002/0471140864.ps0713s60}
}

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Methods for the Design and Analysis of Sedimentation Velocity and Sedimentation Equilibrium Experiments with Proteins

Abstract

Cite this paper

Ultracentrifugation of Macromolecules