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article · Journal of Pharmacy and Pharmacology · 1983

Assay of 6-thioguanine nucleotide, a major metabolite of azathioprine, 6-mercaptopurine and 6-thioguanine, in human red blood cells

Lynne Lennard, John L. Maddocks

Cited by 112Abstract and details only

Abstract

An assay for 6-thioguanine (6-TG) nucleotide, a major metabolite of the cytotoxic drugs azathioprine, 6-mercaptopurine and 6-thioguanine in human red blood cells (RBCs) has been developed. The metabolite was not detected in RBCs when azathioprine or 6-mercaptopurine was incubated with whole blood in-vitro. The assay for intracellular 6-TG nucleotide is specific and requires 8 X 10(8) RBCs (100 microliters packed cells) for which the limit of sensitivity is 30 pmole 6-TG nucleotide. Pre-dose blood samples were obtained, 12 h after the last azathioprine dose, from 10 renal transplant recipients with stable functioning cadaver grafts on a total daily dose of 150 mg azathioprine. The mean 6-TG nucleotide concentration was 171 pmole/8 X 10(8) RBCs (s.d. = 84). The assay is also suitable for use in measuring 6-TG nucleotide in the RBCs of leukaemic children undergoing 6-mercaptopurine treatment.

Cite this paper

APA 7

Lennard, L., & Maddocks, J. L. (1983). Assay of 6-thioguanine nucleotide, a major metabolite of azathioprine, 6-mercaptopurine and 6-thioguanine, in human red blood cells. Journal of Pharmacy and Pharmacology, 35(1), 15–18. https://doi.org/10.1111/j.2042-7158.1983.tb04255.x

MLA 9

Lennard, Lynne, and John L. Maddocks. “Assay of 6-thioguanine nucleotide, a major metabolite of azathioprine, 6-mercaptopurine and 6-thioguanine, in human red blood cells.” Journal of Pharmacy and Pharmacology, vol. 35, no. 1, 1983, pp. 15–18, https://doi.org/10.1111/j.2042-7158.1983.tb04255.x.

Chicago

Lennard, Lynne, and John L. Maddocks. 1983. “Assay of 6-thioguanine nucleotide, a major metabolite of azathioprine, 6-mercaptopurine and 6-thioguanine, in human red blood cells.” Journal of Pharmacy and Pharmacology 35 (1): 15–18. https://doi.org/10.1111/j.2042-7158.1983.tb04255.x.

Harvard

Lennard, L. and Maddocks, J.L. (1983) ‘Assay of 6-thioguanine nucleotide, a major metabolite of azathioprine, 6-mercaptopurine and 6-thioguanine, in human red blood cells’, Journal of Pharmacy and Pharmacology, 35(1), pp. 15–18. Available at: https://doi.org/10.1111/j.2042-7158.1983.tb04255.x.

BibTeX
@article{lennard1983assay,
  title = {{Assay of 6-thioguanine nucleotide, a major metabolite of azathioprine, 6-mercaptopurine and 6-thioguanine, in human red blood cells}},
  author = {Lennard, Lynne and Maddocks, John L.},
  journal = {Journal of Pharmacy and Pharmacology},
  volume = {35},
  number = {1},
  pages = {15--18},
  year = {1983},
  doi = {10.1111/j.2042-7158.1983.tb04255.x}
}

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