article · Microfluidics and Nanofluidics · 2021
Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting
Akihiro Isozaki, Dunhou Huang, Yuta Nakagawa, Keisuke Goda
Cited by 16Open access
Abstract
Abstract Droplet microfluidics is a powerful tool for a diverse range of biomedical and industrial applications such as single-cell biology, synthetic biology, digital PCR, biosafety monitoring, drug screening, and food, feed, and cosmetic industries. As an integral part of droplet microfluidics, on-chip multiplexed droplet sorting has recently gained enthusiasm, since it enables real-time sorting of single droplets containing cells with different phenotypes into multiple bins. However, conventional sorting methods are limited in throughput and scalability. Here, we present high-throughput, scalable, multiplexed droplet sorting by employing a pair of sequentially addressable dielectrophoretic arrays (SADAs) across a microchannel on a microfluidic chip. A SADA is an on-chip array of electrodes, each of which is sequentially activated and deactivated in synchronization to the position and speed of a flowing droplet of interest. The dual-SADA (dSADA) structure enables high-throughput deflection of droplets in multiple directions in a well-controlled manner. For proof-of-concept demonstration and characterization of the dSADA, we performed fluorescence-activated droplet sorting (FADS) with a 3-way dSADA at a high throughput of 2450 droplets/s. Furthermore, to show the scalability of the dSADA, we also performed FADS with a 5-way dSADA at a high throughput of 473 droplets/s.
Cite this paper
Isozaki, A., Huang, D., Nakagawa, Y., & Goda, K. (2021). Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting. Microfluidics and Nanofluidics, 25(4). https://doi.org/10.1007/s10404-021-02432-z
Isozaki, Akihiro, et al. “Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting.” Microfluidics and Nanofluidics, vol. 25, no. 4, 2021, https://doi.org/10.1007/s10404-021-02432-z.
Isozaki, Akihiro, Dunhou Huang, Yuta Nakagawa, and Keisuke Goda. 2021. “Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting.” Microfluidics and Nanofluidics 25 (4). https://doi.org/10.1007/s10404-021-02432-z.
Isozaki, A. et al. (2021) ‘Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting’, Microfluidics and Nanofluidics, 25(4). Available at: https://doi.org/10.1007/s10404-021-02432-z.
@article{isozaki2021dual,
title = {{Dual sequentially addressable dielectrophoretic array for high-throughput, scalable, multiplexed droplet sorting}},
author = {Isozaki, Akihiro and Huang, Dunhou and Nakagawa, Yuta and Goda, Keisuke},
journal = {Microfluidics and Nanofluidics},
volume = {25},
number = {4},
year = {2021},
doi = {10.1007/s10404-021-02432-z}
}Made from the index record. Check names, capitalisation and page numbers against the paper. Need another style? AI for Research has over 10,000.